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Having shown the ability of rIciA to inhibit helix opening in-vitro, experiments were designed to assess the ability of rIciA to actually inhibit DNA replication by using a reconstituted replication system.
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Milligrams of extracted HMOs were then reduced, lyophilized, weighed using a microbalance, reconstituted in Nanopure water, and analyzed.
The growth behavior of a small crack was monitored using a plastic replication technique.
The surface roughness of a micro-dimple also includes the replication of the tool edge radius profile, which can be determined by using a replication technique.
For this purpose, we developed an in vitro approach using a recently characterized reconstituted human epidermis (RHE) model engineered from hair follicle.
Using a nuclease module reconstituted with human subunits, we demonstrate that both nuclease subunits are required for deadenylation.
The ability to reconstitute replication with purified proteins would provide a powerful approach to study this process.
They did not use a replication cohort.
Here we address the above issues using p7 reconstituted in bicelles that mimic a lipid bilayer.
DNA replication was investigated by searching for the putative genome replication origins using a bioinformatic tool.
In-situ Ksat was measured using a constant head permeameter with five replications.
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