TiO2 and TiO2 Al composite coatings were prepared by plasma spraying using a reconstituted nanosized feedstock via a spray drying method.
Recent progress in the field of genetic code reprogramming using a reconstituted cell-free translation system has made it possible to incorporate a wide array of non-proteinogenic amino acids, including N-methyl-amino acids and D-amino acids.
Here, we present experiments using a reconstituted fusion system that suggest a simple model in which the complexin accessory helix forms an alternative four-helix bundle with the target-SNARE near the membrane, preventing the vesicle-SNARE from completing its zippering.
Next, we tested the ability of these fusion proteins to inhibit the polymerase activity of both FluA and FluB using a reconstituted viral ribonucleoprotein complex and a reporter gene.
Having shown the ability of rIciA to inhibit helix opening in-vitro, experiments were designed to assess the ability of rIciA to actually inhibit DNA replication by using a reconstituted replication system.
Cell invasion assay was examined using a reconstituted extracellular matrix (Matrigel; BD Biosciences).
Using a reconstituted system, we demonstrate that complexin and synaptotagmin simultaneously bind to neuronal SNARE complexes and that both apo-synaptotagmin and complexin inhibit SNARE-mediated membrane fusion.
To test whether they are sufficient, we used a reconstituted import system containing recombinant NTF2, RanGDP, RanGAP, RanBP1 and importins.
To explore the effect of cotransin on TMD integration, we used a reconstituted system comprising a mammalian translation extract supplemented with microsomal ER membranes (Sharma et al., 2010).
mAb, monoclonal antibody; pAb, polyclonal antibody To determine the subcellular localization of ΔCD98-βgeo fusion protein, we first used a reconstituted frog oocyte system.
Here, we used a reconstituted proteoliposome system to accurately measure reversal potentials for peptide-coupled proton fluxes and found that di- and tri-peptides are transported using different proton stoichiometries.
