Milligrams of extracted HMOs were then reduced, lyophilized, weighed using a microbalance, reconstituted in Nanopure water, and analyzed.
We measured the rate of lysine incorporation using a previously described reconstituted E. coli translation system (Youngman et al., 2004; Gromadski et al., 2006; Zaher and Green, 2009) on a series of lysine-encoding simple mRNAs including: AUG-AAA-UUC-AAG-UAA (MKFK-Stop), AUG-UUC-AAA (MFK), AUG- AAA or AUG- AAAA (MK(A or G)5-Stop).
Using a nuclease module reconstituted with human subunits, we demonstrate that both nuclease subunits are required for deadenylation.
Here we address the above issues using p7 reconstituted in bicelles that mimic a lipid bilayer.
E. coli lyfo-disks were reconstituted by crushing one pellet using a sterile spatula in 0.5 mL of sterile TSB.
In a first step, the overall effect of D2O on in vitro gene expression rates was studied by using a commercial prokaryotic cell-free gene expression system reconstituted of purified components from E. coli and a T7 RNA polymerase [ 20, 23], where H2O was replaced by D2O.
An aox1 mutant strain was created using targeted gene disruption, and the mutant strain was reconstituted to wild type using a full-length AOX1.
Make fruit smoothies using the reconstituted milk.
When E. coli begins using a gene to make a protein, it does not produce a smoothly increasing supply.
Mixing is facilitated using a supplied system which is most important in reconstituting the fibrinogen component.
PepTSt was reconstituted into Escherichia coli total lipids with egg PC liposomes and assayed using a proton-driven system as previously described [ 3].
