Sentence examples for using a recombinant substrate from inspiring English sources

For example, in vitro γ-secretase activity is measured routinely using a recombinant substrate derived from APP [23].

The difference in Notch processing might be explained by the fact that they used a recombinant substrate whereas we studied the processing of endogenous Notch.

Total whole brain membrane fractions from 1, 12, 18, and 24 month old C57BL/6 mice were assayed for in vitro Notch1 γ-secretase activity using a recombinant Notch1 substrate.

Total whole brain membrane fractions were isolated from male and female C57BL/6 mice at 1, 12, 18, and 24 months of age and in vitro γ-secretase activity was measured using a recombinant APP substrate.

The IP-IKK complex was analyzed in an in vitro kinase assay using a recombinant GST-IκBα protein as substrate both in the absence and in the presence of GlcN and NAPA.

Cyp-2E1 activity was assessed in a cell-free system, using a recombinant Cyp-2E1 and a specific substrate [44].

Here, we describe a reconstitution of PBP2 reaction by using a recombinant S. aureus enzyme and its substrates, lipid II (wild-type) and depsi-lipid II analogues (VRSA).

It remains unclear whether this approach reflects the true pharmacodynamic properties of Plk1 inhibitors, since these assays often address only small portions of the human kinome and typically use a recombinant kinase and an artificial substrate.

Using in vitro recombinant substrate proteins, chaperone assays have indicated that αB-R120G reduces or abolishes chaperone function, becomes unstable and prone to aggregation and insolubilization with time, and exists as a large oligomer with a molecular mass twice that of wild-type αB-crystallin [31], [34].

Also, consider using a different substrate.

The activity of purified recombinant PikI was measured using a range of substrates.