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We investigated whether the metacyclic trypomastigote stage-specific surface molecule gp82, a Ca2+-signal-inducing molecule implicated in host cell invasion, displayed the ability to induce actin cytoskeleton disruption, using a recombinant protein (J18) containing the full-length gp82 sequence fused to GST.
The investigators of this study showed the enzyme was biochemically active using a recombinant protein.
Interestingly, dimerization of LevQ could also be observed in vitro using a recombinant protein (Figure S6).
In this context, we have recently produced several mouse mAbs [24], using a recombinant protein corresponding to the C-terminal binding domain of Botulinum neurotoxin A1 (Fc-BoNT/A1, 50 KDa) which has protective antigenic properties [25].
With this type of assay, we also avoid the use of viral particles by using a recombinant protein, the HA fragment of the 2009 H1N1 influenza virus (specific details will be provided later) produced in E. coli as the antigen of an ELISA method.
The activity was characterized using a recombinant protein produced by Pichia pastoris.
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To confirm the direct interaction, we used a recombinant protein.
Burbelo and colleagues used a recombinant protein expressed from the helicase domain of CHV NS3 as antigen to detect CHV antibodies in sera from different animals.
We used a recombinant protein fragment consisting of glutathione S-transferase (GST) fused to this SH3SH2 region of Arg (GST-Arg-SH3SH2) to affinity purify interacting proteins from extracts of whole mouse brains.
The bead-based assay used a recombinant protein heterotrimer that was heavily skewed toward A2B BLyS/APRIL trimers as a reference standard, but nevertheless, was also able to detect AB2 trimers.
Yang et al. captured proteins from RD cells lysates using a recombinant EV71 Vproteinein fused to a calmodulin-binding protein tag.
More suggestions(15)
using a recombinant p2T7
using a recombinant vaccine
using a recombinant suicidal
using a HiTrap protein
using a recombinant T-cell
using a recombinant chitin
using a Subcellular protein
using a recombinant cyanide
using a commercial protein
using a recombinant hemagglutinin
using a green protein
using a recombinant version
using a recombinant substrate
using a total protein
using a recombinant yeast
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