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We tested for local adaptation at two different scales, both within (ca. 10 80 km) and between (ca. 3300 km) regions, using a reciprocal inoculation experiment in the greenhouse for nine months.
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CNV calls were clustered into CNV loci using a reciprocal overlap threshold (Figure 3).
The skin was homogenised using a reciprocal shaker (Fisher Scientific UK) for 45 second (6.5 speed).
Orthologs were identified using a reciprocal best hit approach.
We calculated a set of orthologs using a reciprocal best BLASTP criterion for these genes.
Orthologs present in both species transcriptomes were identified using a reciprocal BLAST approach (see above).
Cognate rainbow trout expressed sequence tags (ESTs) were subsequently identified using a reciprocal blast search strategy.
InParanoid automates the identification of orthologs using a reciprocal BLAST based approach.
Orthologues of protein coding genes were identified using a reciprocal best blast hit (RBH) strategy.
Latency to reach the food data were right skewed and were transformed using a reciprocal transformation.
To find orthologous sequences present in both species sets, we used a reciprocal BLAST approach [ 98].
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