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Here, we grouped the proteins predicted from S. sulcatum transcriptome with four ciliate genomes (Oxytricha trifallax, Tetrahymena thermophila, Ichthyophthirius multifiliis and Paramecium tetraurelia) using a reciprocal BLAST hit (RBH) approach.
This makes it more difficult to successfully crosscheck hits using a reciprocal BLAST as the number of unknown matches returned as top hits increases.
Orthologs present in both species transcriptomes were identified using a reciprocal BLAST approach (see above).
Cognate rainbow trout expressed sequence tags (ESTs) were subsequently identified using a reciprocal blast search strategy.
InParanoid automates the identification of orthologs using a reciprocal BLAST based approach.
Those murine ESTs were subsequently used to identify cognate rainbow trout (Oncorhynchus mykiss) ESTs using a reciprocal Blast search strategy.
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Orthologous relationships between genes were defined in the initial comparative genomic analysis [ 7] using a reciprocal BLASTing method and this information is available via the T. annulata genome browser [ 19].
To identify GSK3 homologs we used a reciprocal blast strategy: nucleotide sequences of Arabidopsis GSK3 genes [ 10] were first used to seed tblastx searches to identify potential GSK3 homologs in the above sequence data bases, and these were next used as queries in tblastx searches of all Arabidopsis genes.
Sequences were compared using a reciprocal-BLAST-like setting where each sequence is used both as query and database entry.
Orthologues of protein coding genes were identified using a reciprocal best blast hit (RBH) strategy.
Ortholog prediction is critical for comparative analyses and annotation transfer across species and is typically performed on a genome-scale using a reciprocal best BLAST hits (RBBH) approach.
More suggestions(15)
using a relaxed blast
using a reciprocal co-immunoprecipitation
using a reciprocal titer
using a classical blast
using a reciprocal introgression
using a reciprocal assay
using a full blast
using a reciprocal shaker
using a similar blast
using a reciprocal theorem
using a reciprocal interval
using a second blast
using a specialized blast
using a raw blast
using a reciprocal inoculation
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