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The inversion was detected in three European individuals (Fig. 3C) and inversion genotype status was confirmed using a reciprocal assay (Fig. 3D).
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Using a reciprocal immunoprecipitation assay, we demonstrated that full-length Smo interacts with several BBSome subunits with BBS1 showing the strongest interaction (Fig. 2A and Supplementary Material, Fig. S1D).
Using a reciprocal co-immunoprecipitation assay, our findings show an ER stress-related interaction between TMEM33 and PERK (Fig. 5a).
An evaluation of the functional significance of peat microorganisms using a reciprocal transplant approach.
In Chapter 3, I tested these variants using a reciprocal transplants between warm and cool microclimates.
Clusters were determined using a reciprocal best pair match and then an algorithm for adding in-paralogues was applied.
CNV calls were clustered into CNV loci using a reciprocal overlap threshold (Figure 3).
The skin was homogenised using a reciprocal shaker (Fisher Scientific UK) for 45 second (6.5 speed).
Orthologs were identified using a reciprocal best hit approach.
We calculated a set of orthologs using a reciprocal best BLASTP criterion for these genes.
Orthologues of protein coding genes were identified using a reciprocal best blast hit (RBH) strategy.
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