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Using a reciprocal approach, the algorithm was used to calculate the read length required to produce assemblies with 48, 96, 192, 384, and 762 gaps (gap benchmarks).
The EE unique gene list was generated using a reciprocal approach (i.e., relaxed criteria (P1 t) > 0.9 and |fold change| > ± 1.4 cut-off) to obtain a liberal TAM-mediated gene list which was then excluded from the original EE unique gene list using P1 t) > 0.999, and |fold change| > ± 1.5 criteria).
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Orthologs present in both species transcriptomes were identified using a reciprocal BLAST approach (see above).
Data sets of orthologous gene pairs between BFo strains and E. tasmaniensis and E. pyrifoliae were constructed using a reciprocal BLAST approach.
Using a reciprocal transplant approach, we found evidence for genetically based differences both between and among northern, coastal and southern, inland populations of wild radish.
Identification of aminopeptidase genes related to the S-LAPs in other insects was conducted using a reciprocal blast approach where the eight S-LAPs were first compared to a combined database of RefSeq, non-RefSeq and ab initio coding sequences from A. gambiae, C. quinquefasciatus, A. aegypti, T. castaneum, N. vitripennis, A. pisum and A. mellifera using Tblastx.
The qualitative analyses will incorporate the following processes [ 50, 51]: 1) Invite participants to review the transcripts of interviews, and summarize their perception of the data, for accuracy, and check for the trustworthiness of the data; 2) Conduct a thematic analysis using a reciprocal coding approach where researchers engage in open dialogue about themes and data interpretation.
Orthologs were identified using a reciprocal best hit approach.
InParanoid automates the identification of orthologs using a reciprocal BLAST based approach.
We then generated a consensus transcriptome by identifying orthologous sequences among the four samples using a reciprocal best BLAST approach.
Detection of orthologs was performed using a reciprocal best-hits approach as implemented in the RSD-algorithm [ 37].
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