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Using a recently described skin-humanized model based on the engraftment of human bioengineered skin equivalents onto immunodeficient mice, we compared the efficacy of different in vivo gene transfer strategies aimed at delivering growth factors to promote skin wound healing.
Here, we demonstrate a method for the rapid production of negative control tandem scFvs through complementarity determining region (CDR) mutagenesis, using a recently described bispecific T-cell engager (BiTE) targeting a tumor-specific mutation of the epidermal growth factor receptor (EGFRvIII) as an example.
The covariance matrices were estimated using a recently described shrinkage approach [30], [31].
When these data were adjusted for misclassification, by using a recently described formula [17], the adjusted BED estimates were lower.
Using a recently described inducible shRNA system [29], we have previously shown that signaling via oncogenic BRAF is essential for tumor initiation and maintenance in melanoma models [30].
The same constructs were transfected in HEK293T cells and global levels of genomic hmC were measured using a recently described hmC glucosylation assay [39].
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We used a recently described human experimental cadaveric model to compare the stability of the closures.
To this end, we used a recently described transgenic P. berghei line, P. berghei [PfCS], whose CSP gene was replaced by that of P. falciparum [17].
To analyze whether promoter regions of differentially methylated genes showed different CpG compositions, we used a recently described classification into promoters with high (HCP), intermediate (ICP) and low (LCP) CpG content [28].
We used a recently described murine coculture model consistent of cortical organotypic slice cultures (COSC) and the murine brain endothelial cell line bEnd.3 as documented in detail before [15].
To understand the extent to which the Let7 pathway defines C5 tumours, we used a recently described signalling pathway impact analysis (SPIA) [34] to probe other signalling pathway dependencies.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com