Sentence examples for using a reaction volume from inspiring English sources

The cDNAs were amplified in an Applied Biosystems 7500 Real-Time PCR system using a reaction volume of 7.5 μl containing 1× SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA) and 15 pmol/μL of each primer.

PCRs were carried out in a BIORAD My Cycler tm thermocycler using a reaction volume of 25 μl containing ~ 25 ng genomic DNA, 200 μM each dNTP (Roche Diagnostics), 0.5 μM each primer, 0.625 U Taq DNA polymerase (Roche Diagnostics), the buffer recommended by the polymerase supplier and 2.5 mM MgCl2.

For cDNA synthesis, 100 200 ng RNA was used in a reaction volume of 20 μL using GeneAmp RNA PCR (polymerase chain reaction) Kit (Applied Biosystems, Nærum, Denmark) as recommended by the manufacturer.

Fluorescein-labelled probes were generated using the CDP-star random prime labelling kit (Gene Images); 250 ng of template was used in a reaction volume of 50 μL and incubated for 90 min. Aliquots of 5 μL were used for each Southern blot experiment.

Between 200 and 400 μg recombinant protein dissolved in ESB was used within a reaction volume of 750 μl.

iQ SYBR Green Supermix (BioRad Laboratories, Mississauga, ON, Canada) was used with a reaction volume of 50 μL.

A 6 μl aliquot of cDNA diluted 1/24 with sterilized water was used in a reaction volume of 20 μl per well.

The assay was slightly modified for this experiment by using a larger reaction volume (2 mL total volume), which accommodates multiple sampling over time.

Reverse transcription reaction was done using a final reaction volume of 20 μL containing 1 μg of RNA, 2.5 μM of oligo dT 18, 10 U Transcriptor Reverse Transcriptase and 20 U Protector RNase Inhibitor (Roche) according to the manufacturer's instructions.

following the manufacturer's guidelines in 24- to 34-plex reactions in 384-well plates using a total reaction volume of 5 µl including 12.5 ng of genomic DNA.

Polymerase chain reaction (PCR) was performed in a thermal cycler (Applied Biosystems® Veriti®, California, USA) using a total reaction volume of 10 μl.