Based on Hess's law, thermochemical cycles were designed to determine the dissolution enthalpies of reactants and products using a solution-reaction isoperibol calorimeter at 298.15 K, and the molar reaction enthalpies were calculated on the basis of above dissolution enthalpies.
PCR reactions were performed with Platinum® Pfx DNA polymerase (Invitrogen, Carlsbad, CA) using the reaction solution recommended by the manufacturer.
For staining, wells were washed with 1 mM MgCl2 in PBS, and cells were fixed by 0.5% (w/v) glutaraldehyde at room temperature, then stained using X-gal reaction solution, and incubated at 37 °C until a color change was obtained.
89Zr4+ was produced in aqueous solution through the 89Y p,nuclearnucleareactionon using a solution target containing yttrium nitrate and dilute nitric acid [18].
Real-time PCR was performed using SYBR Premix Ex Taq reaction solution (TaKaRa, Bio. Inc., Kyoto, Japan) and a StepOne Plus real-time PCR system (Applied Biosystems, CA, USA).
The rod- or sheet-like hydroxyapatite (HAp) nanostructured surfaces were controllably constructed via using different hydrothermal reaction solution.
After incubation with the substrate for 60 min at 37°C, the reaction was terminated using a stop solution (0.3 mol/L glycine/0.2 mol/L sodium carbonate, pH 10.7).
When the 1/0.5 mM AgNO3/BuNH2 were used, the reaction solution did not change in color and remained transparent throughout the reaction.
Reactions were developed using a substrate solution (H2O2 containing 3.6 mM 4-chloro-1-naphthol). Differential expression of HCC was observed, with an over expression in recurrent and primary pterygia compared with a normal conjunctiva.
