Sentence examples for using a reaction mixture from inspiring English sources

The methane combustion mechanism was investigated by means of pulsed reaction experiments using a reaction mixture labeled with 18O2.

The yield of gallic acid glucoside was found to be 35.7% (114 mM) by response surface methodology using a reaction mixture of 319 mM gallic acid, 355 mM sucrose, and 930 mU/mL dextransucrase.

The t-Darpp and Darpp-32 mRNA levels were quantified by a SYBR® Green assay, using a reaction mixture purchased from Applied Biosystems (Foster City, CA).

Following cellular RNA extraction using Trizol reagent (Life Technologies , Inc. according to the manufacturer's instructions, 2 µg of total RNA were reverse transcribed using a reaction mixture as previously described [25].

GPx activity was measured by using a reaction mixture consisting of 0.2 mM H2O2, 1.0 mM GSH, 0.14 U of glutathione reductase (GR), 1.5 mM NADPH, 1.0 mM sodium azide, and 0.1 M phosphate buffer (pH 7.4) and 1 mg/ml of supernatant protein [32].

Kinase assays using purified CDK complexes were performed using a reaction mixture of 0.2 ug kinase complex or PKA, 1 ul of 100 mM ATP, and 0.5 ug GST fusion protein diluted to a final volume of 35 ul with kinase buffer.

The qPCR assay used a reaction mixture consisting of the following final concentration: 0.5  μ M of forward and reverse primers, 250  μ M of each dNTP (GE Healthcare, Little Chalfont, UK), 1 × HotStart Buffer (Qiagen), 1.5 m M MgCl2, 1.5 units HotStart polymerase (Qiagen), 2  μ M SYTO 9 (Invitrogen, Life Technologies), and 1 × ROX reference dye (Invitrogen).

An aliquot (5 μl) of template DNA was used in a reaction mixture containing 10 μl 2× Quanti Tect SYBR Green PCR Master Mix, 500 nmol l-1 of each forward and reverse dsrA primer (Dsr1f – 5ACSCACTGGAAGCACGGCGG3'; DsrR– 5'GTGGMRCCGTGCandTTGG3'), and water to a final volume of 20 μl.

For PCR amplification 1U BIOTAQ™ DNA polymerase (Bioline GMBH Germany) was used in a reaction mixture containing 1.6 mM MgCl2.

To construct the complementing plasmid pKK214hfq, the hfq coding region and 191 bp directly upstream were PCR amplified from F. novicida using primers FN_hfqProm-F-SmaI and FN-hfq.orf-EcoRI-R using a standard reaction mixture and cycling parameters.

Unless noted otherwise, enzyme activities were determined using a standard reaction mixture containing 50 mM Tris-HCl (pH 7.4, 37 °C), 100 mM KCl, 10 mM MgCl2, 1 mM DTT, 100 μg/mL bovine serum albumin, 10% glycerol, and 500 nM single-nucleotide gapped DNA.