Here, we extend the optogenetic toolset by developing a neuromodulatory approach using a rationale-based design to generate a Gi-coupled, optically sensitive, mu-opioid-like receptor, which we term opto-MOR.
Starting from the canonical structure of these models (schematic diagram in Figure 1a ), we reduced the signaling network using a rationale-based approach to obtain a more parsimonious description while retaining the essential features of the IL-6 signaling pathway.
By using a rationale-based strategy and a reverse target-identification approach (identifying the target(s) of a compound after unveiling its cellular phenotype or biological activity), we excavated a likely mechanism that attributes to the activation of p53 by this compound, that is inhibition of SIRT1 activity (Fig 8).
It outlines how to develop and use a rationale for imagery in the context of digital interfaces.
Using a similar rationale for the new MgCO3 mineral formation, it would be difficult to distinctly identify the source of Ca2+ ions for the formation of CaCO3.
Unlike parametric alternatives for time series generation, non-parametric approaches generate new values by conditionally resampling past observations using a probability rationale.
We then grouped, using a phylogenetic rationale whenever possible, all sub-haplogroups with frequencies lower than 1.5%, thus allowing a reduction of the overall number of categories from over 39 to 19.
Using a similar rationale to that described for multi-well plate-based assays, substrates can also be mixed.
Using a similar rationale, we counted gains and losses of FSFs in individual proteomic lineages by traveling from the root to terminal leaves of the proteome tree.
Another trend showed that students who began the course using a nodal emphasis rationale when interpreting trees shifted their rationale to focus on implied apomorphies and common ancestry by the end of the course.
