By using a rat cardiomyoblast cell line, H9c2 cells and cultured primary neonatal rat cardiomyocytes, we have determined the effects of GSLs on hypertrophy.
By RECETOX, Brno, dioxin-like toxicity in vitro is investigated using a rat hepatoma cell line H4IIE.luc, which determines dioxin-like action (generated by e.g. PCBs, dioxins, polycyclic aromatic hydrocarbons etc).
The bioactivity of the synthesized GLP-1C was determined using a rat insulinoma cell line, RIN-m5F.
Using a rat macrophage cell line, NR8383, we could confirm that TLR3, but not TLR4, was upregulated after stimulation with pristane, which excludes LPS contamination in the experiment.
To test this hypothesis, we evaluated the activity of the PCB mixture and the individual components in a transient transfection system using a rat somatomamatroph cell line (GH3) transfected with a reporter gene (luciferase) driven by a canonical TH response element (TRE; direct repeat with a four-base spacer, DR4).
Cytotoxicity was determined using a rat skeletal myoblast cell line (L6 cells).
Biological functionality was evaluated using a rat mesenchymal stem cell (RMSCs) culture.
To verify the effect of RA on osteoblast differentiation, AJ18 expression level was examined using a rat clonal preosteoblastic cell line, ROB-C20 (C20).
C5a neutralization assay was done using a rat basophilic leukemia cell-line transfected with the human C5aR.
An in vitro invasion assay using a rat lung endothelial (RLE) cell monolayer revealed that pretreatment of the RLE cells with MT, but not c-SST-2 cells, significantly reduced the invasion of the RLE monolayer by c-SST-2 cells.
