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The PVN and ME, as well as noradrenergic nuclei from the brainstem (A1, ventrolateral medulla; A2, nucleus tractus solitarus; and A6, locus coeruleus) were isolated from 300 µm brain sections via micropunch tools, as described before3,19 using a rat brain atlas20.
MRI images were collected 7 weeks after rats were 6-OHDA unilaterally lesioned and were manually aligned in space using a rat brain atlas [38].
Regions of interest [ROIs] were drawn over the frontal cortex, striatum, locus coeruleus, and cerebellum, which were anatomically identified from the cryomicrotome sections using a rat brain atlas [27].
Using a rat brain slicer (Rodent Brain Matrix, Adult Rat, Coronal Sections, ASI Instruments, USA) 3 mm coronal sections were dissected, cutting at distances of 2, 5, 8, and 11 mm from frontal pole.
For morphometric examination, 2-mm-thick 2-mm-thick 2-mm-thick coronalng a rat brain matrix.
In the present study, we examined the protective mechanism(s) of DADLE against apoptosis using a rat brain slice model.
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We used a rat brain slice preparation with a voltage-sensitive imaging system to compare the electrophysiological characteristics of intra-amygdala pathways.
At least one previous study [ 27] used a rat brain atlas [ 81]; the second report [ 25] did not indicate the method used for identification of different regions.
Electromagnetic field simulations and specific absorption rate and loss return responses were performed using a rat's brain phantom weighing 100 mg.
The present study used a rat neocortical brain slice EEG preparation to investigate excitatory synaptic mechanisms underlying anesthetic-induced burst suppression activity.
In all rats, they acquired individual brain perfusion studies with SPECT (U-SPECT-II) after DBS (stimulator on and off), micro-CT scans and, after the animals were killed and the electrodes removed, MRI scans on a clinical MRI scanner using a dedicated rat brain oil [ 131].
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