Sentence examples for using a rat anti-mouse from inspiring English sources

The following day, macrophages were depleted from the culture by negative selection using a rat anti-mouse FcgRII/III antibody coupled to anti-rat coated magnetic beads (Dynal).

For the detection and quantification of wall-adherent thrombosis, mouse platelets were detected by immunohistological staining using a rat anti-mouse glycoprotein IIb (CD41) polyclonal antibody (Clone MWReg30, GeneTex, USA).

Macrophages were stained using a rat anti-mouse Mac-3 monoclonal Ab (BD Biosciences, dilution 1/75) and revealed with a secondary biotin-conjugated goat anti-rat Ab (Jackson Immunoresearch, dilution 1/200).

Five µm sections were subjected to immunohistochemistry using a rat anti-mouse Ki67 monoclonal antibody that specifically recognizes proliferative cells (DakoCytomation) and a rat anti-ATX monoclonal antibody (clone 4F1).

Intratumoral microvascularity was assessed using a rat anti-mouse CD34 antibody (1 25; Cell Sciences, Canton, MA).

F4/80 staining was completed by the Vanderbilt Translational Pathology Shared Resource using a rat anti-mouse monocolonal antibody against F4/80 (CI:A3-1) (Novus Biologicals).

This assay was specific for IL-12 p70 by virtue of the use of a rat anti-mouse IL-12 (p70) capture antibody (BD Pharmingen, clone 9A5), which reacts with the p70 heterodimer but does not react with the p40 subunit.

To detect microvascular density (MVD) in the peri-infarct area, a rat anti-mouse CD31 was used.

To detect vascular density in the infarct area, a rat anti-mouse CD31 antibody was used.

The presence of platelets was assessed with a rat anti-mouse gpII1/IIIb mAb (CD41, Serotec) and T cells using rat anti-mouse CD3 (KT3, Serotec).

Co-staining using a rat anti-α6 antibody and a mouse anti-β4 antibody showed that the α6 integrin subunits co-localize with the β4 subunit in both shctl (Supplementary Figure 2A– C, available at Carcinogenesis Online) and shα6A cells (Supplementary Figure 2D FF, available at Carcinogenesis Online) with a typical punctuated hemidesmosome-like staining pattern.