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Serum C-peptide levels were measured using a rat C-peptide radioimmunoassay (Linco Research , Inc St. Charles, MO).
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Commercial RIAs (Linco Research) were used to measure human and rat C-peptide [ 25].To assess liver function, aspartate transaminase (AST) and alanine aminotransferase (ALT) were measured daily over the first 10 days and then monthly using commercial kits (Roche Diagnostics, Castle Hill, NSW, Australia).
C-peptide was measured by ELISA in plasma harvested at sacrifice (Rat C-peptide ELISA; ALPCO).
β-Cell responsivity (ΦTotal) was measured using a C-peptide minimal model.
Specific insulin or C-peptide measurements were made using the Liaison C-peptide and insulin tests on a Liaison analyzer (DiaSorin, Saluggia, Italy).
A radioimmunoassay was used to measure C-peptide in DPT-1.
Insulin sensitivity using OGTT glucose and C-peptide (ISOGTT C-pep) was calculated according to the methodology of Radaelli et al. (9), with a numerator adjustment for scaling: ISOGTT C-pep = 1,000/√ FPG × fasting C-peptide × G × C), where G and C are the means of fasting and 1-h PG (mg/dL) and C-peptide (μg/L), respectively.
For studies on protein-peptide conjugates, CPE30 peptide was conjugated to HA antigen using a CPE30 peptide synthesized with a c-terminal GGGGSGGGGS linker.
To identify platelet proteins that interact with the C terminus of SERT, we used a proteomic approach based on peptide affinity chromatography by using a synthetic peptide corresponding to the last 26 amino acids from the C-terminus (586 630) of SERT.
One report used rat SMCs and human C-peptide and saw a modest effect on high glucose-induced (25 mmol/l) proliferation, but only after 3 weeks [ 44].
Therefore, we tested the specificity of the antibody response in rabbits and rats against linear epitopes using a DBL4ε peptide ELISA.
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