This study employed an iterative cycle of library synthesis and screening, using a novel rapid equilibrium dialysis assay, to discover a modified Zn-BDPA structure with high and selective affinity for vesicles containing PS.
More specifically, we have fabricated a 25-member library of modified Zn-BDPA library candidates and tested the affinity of each library candidate for PS-rich membranes using a novel rapid equilibrium dialysis assay.
The GPDH reaction is in a rapid equilibrium, therefore used as the input to the mitochondrial component, replacing pyruvate dehydrogenase (PDH) in the model.
After some experimentation, we developed a rapid equilibrium dialysis (RED) assay using a commercially available apparatus that consisted of two compartments, source and receiver, separated by a microporous dialysis membrane (8000 molecular weight cutoff) that we confirmed was impermeable to 200-nm-diameter vesicles.
The protein binding of GSK-7975A in the blood and pancreas was determined at 1 μg/mL using a dialysis Teflon block (HTDialysis, LLC, Gales Ferry, CT) with dialysis membrane strips and a rapid equilibrium dialysis device (Thermo Scientific), respectively.
Nevertheless, ODC has been shown to undergo a rapid equilibrium between the active dimer and the inactive monomer in vivo and in vitro39,41,42,43.
The first involves a rapid equilibrium between the type II bound substrate and a metabolically oriented binding mode.
The two substrate enzymes were assumed to follow a rapid equilibrium, random, bi-bi mechanism.
The concentration time profiles of brain ECF, CSFLV, and CSFCM indicate a rapid equilibrium with plasma.
This corresponds to a rapid equilibrium assumption for the reaction (21) D [0, p ] + D [ L, E ] ⇋ D [ L, p ] + D [0, E ] which is not part of the reaction scheme and where the equilibrium constant equals one.
