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Human plasma levels of lipocalin 2 (also known as NGAL) were determined using a rapid ELISA kit (BioPorto Diagnostics, Gentofte, Denmark) following the manufacturer's protocol.
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HIV seropositivity was determined either with two complementary enzyme-linked immunosorbent assays (ELISAs) [ 22] or using a rapid testing strategy with Determine (Abbott), followed by Genie II (BioRad), as recommended by WHO [ 23], with an ELISA confirmatory testing for discrepant results.
NGAL was measured using a Bioporto Rapid ELISA [ 28].
Isotypes of the MAbs were determined using a commercial rapid ELISA mouse antibody kit (Thermo Fisher Scientific, Rockford, IL, USA).
In search for options to optimize the rapid laboratory diagnostics of RSV we have compared this NASBA method with a published RT-PCR protocol and a rapid ELISA, the latter both used in our routine procedures for the detection of RSV.
A rapid ELISA was done on whole blood collected by finger prick using DetermineTM HIV1/2 kit (Abbot Laboratories, Japan, and STAT-PAK assay kit (Chembio Diagnostic Systems, USA).
Chex is a rapid ELISA kit for the detection of IgM antibodies in human CSF and serum.
Corticosterone was determined using an ELISA assay (ELISA Kit, Assay pro, USA, #EC3001-1).
In this work we describe a rapid ELISA-based assay that detects secretion of four T3SS effector and translocator proteins.
Hormone content in secretion samples was assayed using an ELISA (SST ultrasensitive ELISA kit; Phoenix Pharmaceuticals , Inc.
Discordant rapid test results were resolved using a third ELISA test (Vironostika; Biomérieux, Ltd., France) as a tie-breaker.
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