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Immunoblotting for Beclin-1 and α-tubulin (loading control) was provided in cell extracts silenced using a random siRNA (Randsi) or Beclin siRNA (Beclinsi).
Immunoblotting for Apaf1, caspase-9 and α-tubulin (loading control) was provided in cell extracts silenced using a random siRNA (Randsi) or Apaf1 siRNA (Apafsi).
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A random number was drawn using a random number table.
Using a random number generator, outpatients were selected at random.
All random draws were made using a random number table.
We note that this vector system can potentially be used to generate a random siRNA library.
siRNA were detected using a random-primed probe radioactively labeled with [α-P]-dCTP (3000 Ci/mmol, PerkinElmer, Waltham, MA, USA).
Such an inverted promoter system also opens the possibility of generating a random siRNA library.
NC siRNA is a random siRNA provided by GenePharma Co., Ltd.
We treated Mel Ju cells with siRNA against cJun (si-cJun) or with a random siRNA (si-ctrl).
We used a random intercept and random slopes model.
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