Sentence examples for using a random primer from inspiring English sources

First-strand cDNA synthesis was carried out with Super Script II RNase H-reverse transcriptase (Invitrogen) using a random primer.

This novel RT-PCR was able to amplify viral sequences from cells infected with only a small number of infectious particles (less than 10 TCID50) at three days postinoculation and was as sensitive as the general RT-PCR using a random primer and the interference and immunofluorescent antibody (FA) methods.

Hybridization probes were radioactively labeled using a random primer labeling method.

Northern blot analysis was performed using a random primer labeled [32P] cyclin D1 probe.

Probes were labeled with biotin using a random primer labeling method.

All probes were labeled with [α-32P]dCTP using a random primer labeling kit (Invitrogen, Carlsbad, CA).

Oligonucleotide 1 and 2 were also used to generate non-radioactive and radioactive probes by utilizing the PCR DIG Probe Synthesis Kit from Roche Diagnostics (Mannheim, Germany) or by α-P]dNTP incorporation using a Random Primers Labeling System (Gibco-BRL, Cergy-Pontoise, France), according to the supplier's protocol.

cDNA clones were selected randomly from the cDNA library, and single-pass sequenced using a random T3 primer on an ABI 3730 Genetic Analyzer (Applied Biosystems, Foster City, USA).

cDNA was synthesized using a random hexamer primer.

Complementary DNA (cDNA) was generated from total RNA using a random hexamer primer following the protocol for Superscript II (Invitrogen, Carlsbad, CA).

First-strand cDNA was synthesized using a random hexamer primer using the mRNA fragments as templates.