Sentence examples for using a random hexamer from inspiring English sources

cDNA was synthesized using a random hexamer primer.

Complementary DNA (cDNA) was generated from total RNA using a random hexamer primer following the protocol for Superscript II (Invitrogen, Carlsbad, CA).

500-1000 ng of RNA was reverse-transcribed with Superscript III reverse transcriptase (Invitrogen) using a random hexamer primer, as per manufacturer's instructions, while a no-RT control was set up in parallel to assess the efficiency of the DNAse treatment.

First-strand cDNA was synthesized using a random hexamer primer using the mRNA fragments as templates.

First-strand cDNA was synthesised from 1  μg of total RNA using a random hexamer primer.

First strand cDNA was synthesized using a random hexamer primer and M-MuLV reverse transcriptase.

The purified mRNA was broken down into short fragments and was applied as a template to synthesize the first-strand cDNA using a random hexamer-primer and reverse transcriptase (SuperScript® II Reverse Transcriptase; Invitrogen, Catalog # 18064014).

cDNA was synthesized according to the Superscript III kit (Invitrogen) by using a random hexamers primer.

The total RNA of each of the virus strains was extracted using TRIZOL reagent (Invitrogen), and cDNA was subsequently synthesized using a random hexamers primer, and then standardized to 1000 ng of total RNA per sample.

cDNA was generated from total RNA using a random primer hexamer provided with the High Capacity cDNA Archive kit (Applied Biosystems, Foster City, CA).

Complementary DNA (cDNA) was generated from 5 μg of total RNA using a random primer hexamer (GE Healthcare) and aminoallyl-dUTP mix (Sigma) following the protocol for Superscript III (Invitrogen).