Sentence examples for using a rabbit reticulocyte from inspiring English sources
Equal amounts of in vitro transcribed RNA from the various constructs illustrated in figure 3A were translated using a rabbit reticulocyte lysate system (Fig. 3B).
0.5 µg of each purified transcript was translated using a rabbit reticulocyte lysate system (Promega, USA), according to the manufacturer's instructions, in the presence of 20 µCi Pro-mix (L- [35S]-methionine and L- [35S]-cysteine (Amersham Pharmacia Biotech) per reaction.
In vitro translation was performed using a rabbit reticulocyte system (Promega) according to the manufacturer's instructions.
[S]-Sir4 was synthesized by using a rabbit reticulocyte lysate transcription/translation system (RRL) spiked with S-methionine.
This value was as high as those using a rabbit reticulocyte lysate for genomes prepared by other methods (6, 16).
XBP1u transcripts lacking a stop codon were translated using a rabbit reticulocyte lysate containing S-labelled methionine and ER-derived rough microsomes (RM).
Since Prep1 can bind both some mRNAs and the 4EHP translation inhibitor, we studied the effect of the Prep1-4EHP conplex on Hoxb4 mRNA translation in vitro using a rabbit reticulocytes lysate translation system.
The pOCT mRNA was translated and labeled with the use of a rabbit reticulocyte lysate system in the presence of [S]-methionine.
Briefly, purified GST fusion proteins immobilized on glutathione Sepharose beads were incubated with the lysate containing 35S-labeled syntaxin 1A [syntaxin 1A translated on the template of in vitro synthesized RNAs using a translation rabbit reticulocyte lysate kit (Promega) according to the manufacturer's instructions] with gentle rocking.
The mRNAs were used as templates for protein synthesis in a rabbit reticulocyte lysate (RRL) with 35S-Met or 35S-Cys as radiolabeled tracers.
A rabbit reticulocyte lysate system (Promega Co). was used to prepare the recombinant proteins of Nrf2, MafK and JDP2, according to the manufacturer's protocol.
