Sentence examples for using a rabbit anti-mouse from inspiring English sources
Total protein was isolated from fecal samples, resolved by SDS-PAGE, and immunoblotted using a rabbit anti-mouse RELMβ Ab (PeproTech) [22].
Detection was performed using a rabbit anti-mouse IgG horseradish peroxidase conjugate.
The presence of murine IgG1 specific for PSA was detected using a rabbit anti-mouse IgG1 AP-conjugated antibody (Life Technologies) and a phosphatase substrate (Sigma Aldrich).
MMP-9 protein was detected using a rabbit anti-mouse MMP-9 antibody at a 1 200 dilution (Santa Cruz Biotechnology, Santa Cruz, CA) followed by Alexa Fluor-594 goat anti-rabbit IgG antibody (1 500).
Immunohistochemical detection was done with a Discovery XT immunostainer (Ventana Medical Systems, Tucson, AZ) using a rabbit anti-mouse TF antibody (Sekisui Diagnostics) followed by a biotinylated anti-rabbit IgG (Vector Laboratories, Burlingame, CA) and an anti-avidin-biotin-peroxidase system.
The primary antibodies used were a rabbit anti-mouse GzmB antibody (1 100; Abcam, Cambridge, MA, USA), a rabbit anti-mouse α-SMA antibody (1 2000; Abcam), rabbit anti-mouse CD31 antibody (1 200; Abcam) and rabbit ant-mouse Ki67 antibody (1 500; Cell Signaling Technology, Danvers, MA, USA).
MAbs against IL-8 receptors (CXCR1 and CXCR2) were used by indirect fluorescence developed with a rabbit anti-mouse antiserum tagged with FITC (Jackson ImmunoResearch Labs, West Grove, PA).
We investigated the ERα/PI3K interaction in the human breast tumour cell line MCF-7 using a rabbit anti-ERα together with a mouse anti-p85 antibody.
The primary antibody incubation was performed using a rabbit anti-c-Fos antibody (diluted 1 400), mouse-anti-BrdU antibody (diluted 1 50), and goat anti-Dcx antibody (diluted 1 50) (all primary antibodies, Santa Cruz Biotechnology, Inc, Dallas, USA), for 3 days at 4°C.
The secondary antibody used was a rabbit anti mouse HRP (DAKO) and this was in turn detected using the ECL Western blotting detection reagent (Amersham Pharmacia Biotechnology).
A secondary antibody reaction was performed by using a biotinylated rabbit anti-mouse antibody (Nichirei Bioscience) for 30 min at RT. Peroxidase-conjugated streptavidin (Nichirei Bioscience) was subsequently used for 30 min at RT, and DAB was used to visualize the binding of the first antibody.
