sTREM-1 in BALF was measured using a quantitative sandwich enzyme immunoassay.
plasma C-reactive protein (CRP) using a quantitative sandwich enzyme immunoassay technique (Quantikine Human CRP Immunoassay R&D Systemss Europe Ltd, Abingdon, UK).
Serum sFas levels were determined using a quantitative sandwich enzyme immunoassay.
Resistin serum concentrations were analysed using a quantitative sandwich immunoassay (ELISA; BioVendor, LLC, Candler, NC, USA).
Soluble ICAM-1 was measured using a quantitative sandwich enzyme-linked immunosorbent assay (R&D Systems, Minneapolis, MN).
Both Apo B100 and PON1 were assayed using a quantitative sandwich enzyme immunoassay technique, while C4 was measured using a quantitative competitive enzyme immunoassay technique.
Levels of sFas were measured at the NYU School of Medicine laboratory using a quantitative sandwich enzyme immunoassay technique according to manufacturer (Quantikine Human sFas Immunoassay, R&D Systems Inc., MN).
VEGF and bFGF levels were determined using a quantitative sandwich enzyme immunoassay technique (Quantikine; R&D System, Minneapolis, MN, USA) and all samples were tested in duplicate.
The following inflammatory markers were measured: plasma C-reactive protein (CRP) using a quantitative sandwich enzyme immunoassay technique (Quantikine Human CRP Immunoassay R&D Systemss Europe Ltd, Abingdon, UK).
Serum concentrations of CAT-354 and anti-CAT-354 were assessed by MedImmune using a quantitative sandwich ELISA based immunoassay performed on the Gyrolab assay platform.
