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TNF-α, IL-6, IL-1β, TGF-β1, IL-17A, and IL-23 levels in lung homogenates were determined using a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) according to the manufacturer's instructions (BD sciences, San Diego, CA, USA).
sTREM-1 in BALF was measured using a quantitative sandwich enzyme immunoassay.
Serum sFas levels were determined using a quantitative sandwich enzyme immunoassay.
Resistin serum concentrations were analysed using a quantitative sandwich immunoassay (ELISA; BioVendor, LLC, Candler, NC, USA).
Soluble ICAM-1 was measured using a quantitative sandwich enzyme-linked immunosorbent assay (R&D Systems, Minneapolis, MN).
plasma C-reactive protein (CRP) using a quantitative sandwich enzyme immunoassay technique (Quantikine Human CRP Immunoassay R&D Systemss Europe Ltd, Abingdon, UK).
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Plasma U-II concentrations were measured using a quantitative sandwich-type enzyme immunoassay UT2 kit (Elx 800 ELISA; Cusabio Biotech, Winooski, VT, USA).
While two of the previous studies [ 7, 8] used sFas ELISA assays (Medical & Biological Laboratories Co., Nagoya, Japan), the present study used a quantitative sandwich enzyme immunoassay technique (Quantikine Human sFas Immunoassay, R&D Systems Inc., MN), a very sensitive and specific assay.
The KL-6 and CC16 levels in plasma were measured using a quantitative colorimetric sandwich enzyme-linked immunosorbent assay kit (Blue Gene, China) in accordance with the manufacturer's instructions.
Also, autoantibodies specific for thyroglobulin were measured using a quantitative indirect enzyme immunoassay based on the sandwich method (antithyroglobulin immunoradiometric assay kit; Immunotech, Prague, Czech Republic).
The serum and SF FSTL1 concentrations were detected using a standard quantitative sandwich ELISA (Groundwork Biotechnology Diagnosticate, San Diego, CA, US) with a detection limit of 100 pg/ml.
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using a quantitative approach
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using a standard sandwich
using a quantitative proteome
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using a honeycomb sandwich
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using a quantitative primer-extension
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