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Telomere length was measured using a quantitative real-time polymerase chain reaction (PCR) method.
Slotte et al. [31] studied gene expression of bone healing in PICF using a quantitative real-time PCR technique [31].
Tumor cells were retrieved by laser-captured microdissection and analyzed for MDR1 and ERCC1 expression using a quantitative real-time reverse transcription-polymerase chain reaction assay.
However, validation of microarray data was performed with six selected genes using a quantitative real-time PCR procedure.
RNA was isolated 48 hours later and the amount of mRNA encoding the viral hexon was determined using a quantitative real-time PCR with primers designed to distinguish hexon transcripts of AdHu5 from those of AdC7.
Using a quantitative real-time PCR (qRT-PCR) assay designed to prime only from the mature miRNA [20], the expression profiles of 157 miRNAs (Table S1) were determined in 100 primary AML specimens specifically chosen to exhibit the spectrum of known karyotypes common in AML (Table 1), with examples of AML French American British (FAB) morphological phenotypes [22] from M1 to M6 (Table S2).
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We also used a quantitative real-time RT-PCR approach, and analyzed all breast tumor specimens for DSC3 expression.
To measure TRECs in samples we have used a quantitative real-time PCR (TAQMAN™) assay to amplify a known proportion of the extracted DNA.
We used a quantitative real-time PCR (rt-PCR) to detect the enteric bacterial counts in blood from patients in the emergency room.
Because we wanted a rapid, robust, high-throughput and highly sensitive assay, we chose to use a quantitative real-time PCR assay instead of pyrosequencing to measure LINE-1 methylation.
We used a quantitative real-time PCR assay to determine the relative levels of CHMP2BIntron5 and CHMP2BΔ10 compared with CHMP2BWildtype transcripts in the frontal cortex of three FTD-3 patient brains (Fig. 1B).
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