Sentence examples for using a quantitative primer-extension from inspiring English sources
Expression of individual miRNAs were determined using a quantitative primer-extension PCR assay (Table S1), as previously described.
Quantitative PCR: miRNA levels were determined using a quantitative primer-extension PCR assay (Raymond et al, 2005).
miRNA expression levels were measured using a quantitative primer-extension PCR assay as described earlier (Raymond et al, 2005; He et al, 2007).
Using a quantitative primer-extension PCR assay we identified miRNAs, including, miR-141, miR-200, and miR-429 whose expression is regulated by c-Myc in ES cells, but not in the differentiated and tumourigenic derivatives of ES cells.
For the analysis we used a quantitative primer extension technique [ 10].
To detect mutations, we used the SNaPshot assay, which is a quantitative primer extension assay that yields ratios of mutated and normal alleles.
To this aim the same RNA preparation that had been employed for microarray analysis was used for determination of rRNA synthesis rates by a quantitative primer extension assay [ 10].
Therminator DNA polymerase catalyzes quantitative primer-extension by the incorporation of two glyceronucleotides, with much less efficient extension up to five glyceronucleotides.
SNP genotyping was performed by KBiosciences using a proprietary primer-extension method.
Quantitative primer extension was performed as described [ 10].
We first identified the transcription start site (TSS) of the human Prep1 gene using a primer extension assay (see Methods).
