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RT-PCR was performed for allelic discrimination using a quantitative fluorescence measurement system.
We conducted detailed analyses of both enzymes in the brain using a quantitative fluorescence microphotometry system, MapAnalyzer (Sutoo et al. 1998).
A more recent study using a quantitative fluorescence assay analyzed 2 cohorts (n=340 and 204) of stage I to IV NSCLC in FFPE tissue microarrays.
We analyzed telomere length in control (ctrl) or prelamin A-hMSCs (pre) from four different bone marrow donors of different ages (Fig. 1A) using a quantitative fluorescence in situ hybridization, HT-Q-FISH [ 25].
To understand the impact of this important class of variants and to uncover new principles of BRCT phosphopeptide interactions, we have studied each of these six variants using a quantitative fluorescence polarization assay to determine the precise effect on peptide binding of each of these variants.
CEA was amplified by PCR using a quantitative fluorescence LightCycler™ (Roche Diagnostics, Mannheim, Germany) in a 20 μl reaction mixture containing 2 μl of LightCycler™ FastStart DNA Master Hybridization Probes (Roche), 3.0 m M MgCl2, 0.5 μ M sense and antisense primers, 0.4 μ M fluorescent probe, 0.2 μ M LC-Red probe and 5 μl of undiluted template cDNA in LightCycler™ capillaries (Roche).
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Thus, we developed a quantitative fluorescence microscopic technique using extensive image analysis that enabled us to obtain quantitative data that clearly showed that PGHS-1 level is an excellent marker of early megakaryocyte differentiation.
All throat swab samples were tested using a fluorescence quantitative RT-PCR assay for EV71, CA16, and pan-enteroviruses.
We used two assays to characterize the biological activity of the released peptides: (i) a quantitative fluorescence-based assay using a group-I S. aureus reporter strain that produces GFP under QS control, and (ii) a biofilm production assay using a wild-type group-I S. aureus strain.
The aim of this study was to examine the association between apoptosis-regulating proteins and clinical outcomes in oral squamous cell carcinoma (OSCC) using the quantitative fluorescence immunohistochemistry (IHC) based AQUAnalysis technique.
Imaging was performed using a TE200-IUC Quantitative Fluorescence Live-Cell and Multidimensional Imaging System equipped with a digital monochrome cooled CCD Roper Coolsnap HQ camera (Roper Scientific, Tucson, AZ).
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