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Overall data were then evaluated using a quantified system.
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The assay was modified to obtain a sensitivity of 50 RNA copies per millilitre and it used a quantified synthetic RNA as external standard.
The RNA was eluted in a final volume of 250 μl, assessed for structural integrity using a Bioanalyser system (Agilent Technologies), and quantified spectrophotometrically using a NanoDrop ND-1000 instrument (Labtech).
Isolated PTP1B (0.5 μM) was incubated with 0 25 μM HOSCN (generated enzymatically using a LPO/H2O2/SCN− system; see the Experimental section) for 5 min at 22 °C (pH 7.4), before assessment of the remaining enzyme activity using the conversion of p-nitrophenyl phosphate into p-nitrophenolate anion, with the latter quantified spectrophotometrically at 405 nm.
Such technical judgment can be applied using a system in which the elements of quality are explicitly identified and quantified (scored).
Consider using a supplemental nursing system.
We finally ask which NaCl concentrations are "appetizing" (or "disgusting") using a photometer-quantified dye-feeding assay.
After that it used a licensing system.
Facebook uses a similar system.
Use a frequency system.
Use a reward system.
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