Sentence examples for using a quality threshold from inspiring English sources

36×106 74 bp reads were trimmed with the script trim-fastq.pl using a quality threshold of 20 and a minimum length of 40.

The reads were trimmed with the script trim-fastq.pl using a quality threshold of 20 and a minimum length of 40.

Genotypes for rs12144309 and rs12566340 were imputed using IMPUTE v0.2.0[19] with default parameters, a 10 Mb window centred on the PTPN22 locus and called using a quality threshold of 0.9.

Adaptor removal and quality trimming was done with Nesoni (http://www.vicbioinformatics.com/software.nesoni.shtml) using a quality threshold of 20.

cDNA FASTQ files were processed with a windowed adaptive trimming tool sickle (https://github.com/najoshi/sickle) using a quality threshold of 32.

Therefore, an implemented cluster analysis was performed using a quality threshold algorithm (QT-clustering), in which we only considered genes with differences in transcript levels between the two genotypes at the three time points, i.e. 20 genes for time 0, 190 genes for time 2 h and 414 genes for time 24 h.

In detail, we used a quality threshold above 1.3, we ignored repetitive DNA sequences (i.e. average entropy below 1.0) and requiring at least one exact match against one of our Mycoplasma genomes.

Study design could have been used as a selection criterion or used to set a quality threshold, however the initial search highlighted a paucity of research and only one of the studies retrieved would have met this criterion.

The raw reads obtained from the sequencing system were trimmed for low quality ends with the software Sickle (3), using a phred quality threshold of 20.

Also right-tail clipping was performed using a minimum quality threshold of 20.

Mapped reads of each strain were filtered using a mapping quality threshold of 30 using SAMtools v1.1 [ 63].