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Once you have established yourself as a seasoned Slashdot user, the system will periodically assign you "moderator" status, a temporary position that carries with it the right to rate other members' comments on a scale of 0 to 5. Users can then browse through Slashdot using a quality filter.
After removing PCR artifacts and duplicates, each bam file was filtered using a quality filter score of 30 by using Samtools [ 81].
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Artifacts can be filtered by using a quality index of each analyzed heartbeat in the BCG signal.
Periodically (after every 10 days), 5 mL samples were withdrawn with the help of a sterilized syringe and filtered using a fine quality filter paper (Whatman, Cat No. 1001917, England).
Genome-wide variations were then examined via SOAPsnp11 and SOAPsv using a conservative quality filter pipeline (Li et al. [2009]), and 307 627 SNPs, 57 372 InDels, and 3 096 SVs were yielded from the 7302R genome (Table 2, Additional files 12 and 3).
Uniquely mapped reads were counted for each transcript on both strands with HTSeq-count [ 59] using a mapping quality filter of 30.
Local BLAST analysis (standalone NCBI BLAST v.2.2.27+) was carried out for each file against M. tuberculosis H37Rv reference genome, minimum supporting read depth used to as a quality filter (10 for isolates with >500 fold coverage, 2 for the remaining).
The raw data from the MiSeq run were first processed through a quality filter using established guidelines (Bokulich et al., 2013).
Finally the reads with at least 90% bases > quality 20 were chosen using fastq quality filter for further assembly.
We only kept the high-quality reads using FastX quality filter with the following criteria: minimum of 20 Phred score over at least 80% of the sequence read.
Low quality bases with Phred score <20 corresponding to a probability of error higher than 0.01 [ 33] as well as reads containing unknown bases were removed using FASTQ Quality Filter tool of FASTX-toolkit (http://hannonlab.cshl.edu/fastx_toolkit/).edu/fastx_toolkit/
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