formic acid (50 50 v/ v) by nano-electrospray ionisation MS using a quadrupole-ion mobility spectrometry-orthogonal time-of-flight spectrometer (Synapt HDMS, Waters, Manchester, UK).
For structure identification of the metabolites, collision induced dissociation spectra were recorded by selecting the precursor ion using a quadrupole analyzer (transmission window 2 Da) and the product ions were resolved by the TOF analyzer.
The reaction product ions are mass analysed using a quadrupole mass spectrometer and detected by a secondary electron multiplier (SEM).
Mass spectra of the protein complexes were acquired using a quadrupole time-of-flight (Q-TOF) mass spectrometer equipped with a Z-spray ion source (Q-TOF Premier, Waters, Milford, MA).
In this "tandem-in-time" mode, the fragment ions were produced and trapped in the same trap unit where the precursor ions were trapped and isolated, just like using a single quadrupole ion trap.
LC-MS/MS analyses of in-gel chymotrypsin digested protein bands [ 53] were carried out using a linear quadrupole ion trap ThermoFinnigan LTQ mass spectrometer (San Jose, CA) equipped with a Michrom Paradigm MS4 HPLC, a SpectraSystems AS3000 autosampler, and a nanoelectrospray source.
Analysis of the recovered peptides by nano LC-MS/MS using a linear quadrupole ion trap mass spectrometer and a database search identified the band with 99% probability as Nicotiana endochitinase (GenBank Accession No P24091; EC 3.2.1.14; - Fig. 5B, Table 1).
The ion energy distributions (IEDs) were measured using an electrostatic quadrupole ion energy and mass spectrometer (HIDEN EQP 300).
(2009) examined BCMs in cheese and milk using a nanoelectrospray quadrupole ion-trap mass spectrometer providing a fast and easy means of analysis of these compounds in different matrices.
All mass spectra were acquired using a quadrupole-time of flight (Q-TOF) hybrid mass spectrometer Q-TOF Ultima from Micromass Manchesterr, UK) equipped with a nano Zspray source operating in a positive ion mode.
