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The serum sensitivity of the BbCRASP-2 mutant was measured using a published procedure [16].
using a published procedure [ 25].
All SRPK1 constructs were purified by Ni-resin affinity chromatography using a published procedure.
DNA was extracted from rat kidneys by using a published procedure based on a phenolchloroform-isoamyl alcohol-extraction method (21).
3-Hydroxydecanoyl N-acetylcysteamine (3-OH decanoyl-NAC) was synthesized using a published procedure by coupling -3-hydroxydecanoic aChemicals Chemicals) with NAC (Sigma-Aldrich).
The amount of SWNT in the scaffolds after 12 and 20 days of the degradation process was determined using a published procedure with minor modifications.
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Lung schistosomula were isolated using modifications of a published procedure [ 60].
Metabolite extractions were performed using a modification of a published procedure for Fusarium species (Lowe et al. 2010).
Using a modification of a published procedure [ 23], we designed and synthesized an isothiocyanate molecule that contained a biotin moiety, termed Bio-ITC, to enable specific detection and potential affinity purification of targets of covalent ITC modification.
In order to separate fibres from the ovules, cooled glass beads were combined with the ovules and mechanically agitated under liquid nitrogen, using a modification of a published procedure [ 73].
Anti-FITC antibodies were induced in rodent models with experimental arthritis by vaccination with KLH-FITC (KLH-FITC molar ratio of 1 13), using a modification of a published procedure [ 26].
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