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All experimental procedures involving animals were conducted using a protocol reviewed and approved by the Ethics Committee of Tangdu Hospital, Fourth Military Medical University (Permit Number: 2012028).
Children of both sexes between the ages of 10 and 11 years were recruited by advertisement in the Cambridge, Massachusetts, and Portland, Maine, areas from February 2011 to September 2011 using a protocol reviewed and approved by the Forsyth Institutional Review Board.
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Animals were used in accordance with a protocol reviewed and approved by the Institutional Animal Care and User Ethical Committee of the UMS006 and Région Midi-Pyrénées (Approval#13-U1037-JES08). NSG mice were produced at the Genotoul Anexplo platform at Toulouse (France) using breeders obtained from The Charles River Laboratory.
Animal use and welfare adhered to the NIH Guide for the Care and Use of Laboratory Animals following a protocol reviewed and approved by the IACUC.
All experiments were conducted using a protocol (approval number 970603 to CCL) reviewed and approved by the Institutional Animal Care and Use Committee (IACUC) of National Yang-Ming University.
The tissue samples were frozen surgical specimens obtained using a protocol approved by the institutional review board of The University of Texas MD Anderson Cancer Center.
A systematic review was conducted using a protocol developed a priori based on the Cochrane Handbook for Systematic Reviews of Interventions [ 19].
Leukopheresis units were obtained from volunteer donors using the procedures outlined in our approved human use protocol, reviewed by the established Institutional Review Board at WRAIR.
Both preparations had less than 0.1 EU/ml as determined by using the Limulus Amebocyte Lysate (LAL) QCL-1000 assay kit (Cambrex Bio Science Walkersville, MD) Leukopheresis units were obtained from volunteer donors using the procedures outlined in our approved human use protocol, reviewed by the established Institutional Review Board at WRAIR.
All murine experiments were carried out using protocols reviewed and approved under license by the United Kingdom Home Office under the Animal (scientific procedures) Act 1986.
Medical records were reviewed using a standardised protocol.
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