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In order to establish the cell-type specific patterns we performed miRNA expression profiling on cultures of pure NT2-N neurons expressing neuron-specific enolase (ENO2) and NT2-A astrocytes expressing GFAP produced using a protocol established in our laboratory [32], [44].
Genomic DNA was extracted from the collected specimens using a protocol established for tissue banking.
During this colonization stage, haustorial cells can be isolated using a protocol established by Hahn and Mendgen [ 21].
This pre-sterilization treatment was followed by complete sterilization in the laminar airflow cabinet using a protocol established by Kodja et al. [ 46].
After frozen tissue was ground under liquid nitrogen using a Retsch mixer mill 200 (Retsch Limited, Leeds, UK), genomic DNA and RNA were extracted as above using a protocol established for human tissue.
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Genomic DNA and RNA were then extracted from the ground tissue samples using an EZNA DNA/RNA Isolation Kit and a protocol established for human tissue (Omega bio-tek, R6731-02).
Cell migration was determined using wound-healing assay with a protocol established in our laboratory (Wang et al, 2012a).
The specific heat capacity was determined using a standard protocol established by American Standard Test Method (ASTM E1269) [24].
using a standardized protocol (established and implemented for this and for other postmortem MRI studies by L.A.S.L. and M.L .. MRI scans were acquired on a 1.5 T GE Signa Imager General Electricc, Milwaukee, USA).
Cell cultures of urothelium and associated urinary tract stroma were established using a protocol described by Southgate et al. [17].
The neuronal cells were stained using a immunofluorescence staining protocol established in Moore Lab, EPFL.
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