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Using a protocol adapted by clinic policy fellow Talia Sechley MEM '16 to suit smaller, inland cities, students put on waterproof boots and sampled litter from streams around Durham and Duke, cataloguing the types and locations of their finds.
A paired-end RAD library was constructed using a protocol adapted from Baird et al. (2008).
Ribosome containing lysates were prepared using a protocol adapted from Arava, et al., 2003 [44].
Paraffin sections of embryonic lungs were hybridized using a protocol adapted from [45].
DNA was transfected into cells by electroporation using a protocol adapted from Maniatis.
Artificial but realistic low-resolution data were synthesized using a protocol adapted from Schröder, Levitt and Brunger [8].
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A custom 2.1 M probes sequence capture Nimblegen (Roche NimbleGen, Inc., Madison, USA) microarray designed from those contigs was used with a protocol adapted from Albert et al. [ 90].
For induction of chronic homologous CIA, 8-week-old DBA/1 (H-2q) male mice were used (Harlan UK Limited) and immunized following a protocol adapted from the previously described model [ 27], using an intradermal injection at the base of the tail of 100 μg murine type II collagen emulsified in Freund's complete adjuvant followed by a boost of 100 μg of murine type II collagen given at day 14.
Clontech's SMART cDNA Library Construction kit (Clontech, USA) was used for cDNA synthesis with a protocol adapted by K.V. Donohue (personal communication), and provided by the Genomic Sciences Laboratory, North Carolina State University.
MEFs were transfected with cDNAs encoding human Fcγ receptor FcγRIIA (65) using an Amaxa nucleofector (Lonza AG) and a protocol adapted from (66).
The capacity of the CCTα and CCTδ-KO cells to reciliate after deciliation was investigated using a deciliation protocol adapted to a small number of cells (see Materials and Methods S1 and References S1).
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