Sentence examples for using a protein sequencer from inspiring English sources
The protein blotted on the PVDF membrane was used for determining the N-terminal amino acid sequence using a protein sequencer (Shimadzu PPSQ-31A).
The amino acid sequences of the synthetic peptides were confirmed by sequence analysis using a protein sequencer PPSQ-21A (Shimadzu, Kyoto, Japan), and mass spectrometry using an AXIMA-CFR plus mass spectrometer (Shimadzu).
The purified proteins were transferred to a polyvinylidene difluoride membrane (Applied Biosystems), stained, excised, and subjected to automated Edman degradation using a protein sequencer (Procise 491 HT Protein sequencer, Applied Biosystems).
The sequence of the N-terminal amino acids of purified rhTyrRS was determined using a protein sequencer (PPSQ-33A, USA).
The membrane was stained with Ponceau S solution, and the Nrf1-specific bands (p120 and p110) were excised and sequenced using a protein micro-sequencer (Procise cLC 492A sequencer; Applied Biosystems, Foster City, CA).
After CBB staining, the N-terminal amino acid sequence of the protein was analyzed using a 476A Protein sequencer (Perkin Elmer Applied Biosystems).
Try using a protein treatment.
Attempts at Edman sequencing using a Procise Protein Sequencer to identify the N-terminus were unsuccessful suggesting that it was chemically modified.
The peptides were then sequenced using a Procise™ protein sequencer (Model 491 HT; Applied Biosystem, Warrington, UK) in the gas phase mode from Biobrene™-treated glass fiber discs.
Endotoxin levels in the recombinant proteins were less than 0.05 EU/ml using the Cambrex Limulus Amebocyte Lysate (LAL) pyro Plus Gel Cot assay (lonza, Basel, Switzerland) Purified rPTX3 and rS100A12 were blotted onto polyvinylidine fluoride (PVDF) membrane (Problot, Applied Biosystems) and their N-terminal amino acid sequences were determined using a Procise Protein Sequencer (Applied Biosystems).
The sequence of peptides was analyzed by the Edman method using a 491 Protein Sequencer (Applied Biosystems, Foster City, CA, USA).
