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The purity of the eluted fusion protein was analyzed through 12.5% SDS-PAGE, and the protein concentration was determined using a Protein Quantification Assay Kit (MACHEREY NAGEL, Düren, Germany).
Protein was quantified using a protein quantification assay kit (Bio-Rad Laborathenesubjecteden subjectod to Western blot analysis.
Protein preparations from A549 cells treated with high- and low-binding bacterial strains (MOI = 50 bacteria/cell) were quantified using a protein quantification kit (BioRad).
The cell lysate was centrifuged at 14,000 ×g for 5 min, and the protein in the supernatant was quantified using a Protein Quantification Kit-Rapid (ATTO).
The homogenates were centrifuged at 20 000 × g for 10 min, and the concentration of the soluble proteins was measured by Bradford protein assay using a Protein Quantification Kit (Dojindo, Kumamoto, Japan).
To determine if λ-Exo was covalently attached to the activated PMMA surface of the IMERs reactor bed, we performed spectrophotometric analysis (660 nm) using a protein quantification kit.
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The proteins were quantified using a commercial protein quantification kit (Protein Assay, Bio-Rad) following the manufacturer's instructions.
Protein quantification was performed using a protein assay kit (DC Universal Protein Assay Kit; Bio-Rad, Hercules, CA, USA), and protein integrity was confirmed by SDS-PAGE.
Quantification was performed using a protein assay (Bio-Rad Laboratories, HerCAles, CA, USA).
Normalization of the samples used for immunoblotting was performed using a Pierce protein quantification assay (BCA Protein Assay; Thermo Fisher Scientific, Bonn, Germany).
Protein concentrations in lysates were measured using a BCA protein quantification kit (Pierce, Rockford, IL) using BSA as a standard.
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