Sentence examples for using a protein engineering from inspiring English sources

Decreasing the Km of this enzyme using a protein engineering approach would be a good target for improving isopropanol production and avoiding acetate accumulation in the culture medium.

Using a protein engineering Φ-value analysis to probe the mechanism of formation of this tetramer domain, we infer that the domain folds by the docking of the intrinsically disordered β-spectrin tail onto the more structured α-spectrin tail.

We have compared the folding trajectories of the three-state RNase H and the two-state RNase H, proteins with the same native-state topology but altered regional stability, using a protein engineering approach.

Vedha et al. (2006) used a protein engineering method to create a highly stereoselective NADP-dependent D-amino acid dehydrogenase with broad substrate specificity from C. glutamicum meso-DAPDH and showed its application for one-step synthesis of D-amino acids from their oxo analogs.

use a protein engineering approach to render a temperature-insensitive voltage-gated channel cold- or heat-responsive to reveal principles for temperature-gating and a plausible model for molecularly enabling this mode of environmental responsiveness.

In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.

In this study, to improve the enantioselectivity of Est25, we used a protein engineering approach that involves building a homology model of Est25, library generation based on the model, screening for esterase activity, and screening for specificity toward (S -ketoprofen ethyl eS -ketoprofen

In this study, we used a protein engineering approach; rigidify flexible sites, to improve stability of cABC I. B-factor analysis showed a flexible loop at the N-terminal domain of cABC I which may be involved in its thermal instability and five residues in this region were replaced with proline.

Phage display can be used as a protein engineering tool to select proteins with desirable binding properties from a library of randomly constructed mutants.

Multimerization has been used as a protein engineering tool to increase the number of binding units and thereby enhance the efficacy and retention time of antibody fragments.

In general, site-specific modification first requires the use of a protein engineering method to endow the desired conjugation site with unique reactivity.