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The protein concentration was determined using a Protein Assay Kit (Bio-Rad, Hercules, CA).
Protein contents were determined by the Bradford method (Bradford 1976) using a protein assay reagent (Bio-Rad, USA).
The total protein concentration of the liver homogenate samples were measured using a Protein Assay Kit (Bio-Rad, Hercules, CA, USA).
The protein concentration was measured using a protein assay bicinchoninate kit (Nacalai Tesque), with bovine serum albumin used as a standard.
Protein concentrations of cell lysates were measured using a Protein Assay Kit II (BIO-RAD).
Protein levels of each sample were quantified using a protein assay kit (Pierce Biotechnology, Rockford, IL).
Total protein in the mitochondrial suspension was estimated using a protein assay kit (Pierce, Rockford, IL).
Protein concentrations were estimated using a protein assay kit (Bio-Rad) with bovine serum albumin as the standard.
The protein concentration of the supernatant was determined using a protein assay kit Bio-Rad Laboratoriess, CA, USA).
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The supernatant was collected, and the protein concentration was quantified using a protein assay reagent (BCA, Beyotime, Shanghai).
The total protein was determined using a protein assay reagent (Bio-Rad Laboratories).
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