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Further evidence of the binding of HAMLET to proteasome subunits was obtained using a protein array.
Preliminary analysis of the MSC secretome using a protein array screen revealed a large fraction of chemotactic cytokines, or chemokines.
Cytokine release was evaluated using a protein array that included 42 cytokines.
To identify the apoptotic pathway(s) activated by PA, we performed a comprehensive proteomic analysis using a protein array with antibodies against 43 apoptosis-related proteins.
For CaM binding prediction, we used a dataset of 236 proteins experimentally determined to bind CaM using a protein array screen that tested around a thousand proteins in Arabidopsis thaliana (Popescu, 2007).
Slides were blocked over night and target proteins were detected with specific primary antibodies (Additional file 1, Table 3) using a protein array incubation chamber (n1-quadrat, Metecon, Mannheim, Germany).
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We used a protein array in which different cytokines were quantified simultaneously.
Tarrant et al. used a protein array to screen for protein substrates of O-GlcNAcylated and/or phosphorylated CKII [ 43].
Therefore, to identify the apoptotic pathway activated by PA in our cell model, we used a protein array approach to identify the apoptotic pathways activated by PA.
In an attempt to characterize the molecules released by muscle cells present in the conditioned media (CM), we used a protein array that was able to detect several cytokines and chemokines (Additional file 1: Figure S1).
We used a protein array that contained 265 domains found in chromatin associated proteins [ 78], many of which are known methyl-lysine binding domains (including TUDOR domains, PhD domains, and chromo-domains) or acetyl-lysine binding domains (including bromo-domains, for a complete list see Fig. 6, lower panels).
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