Then, either by sequencing long concatamers of tags using conventional sequencer (SAGE), or by performing iterative parallel sequencing using a proprietary technique (MPSS), the identity of a sufficiently large amount of tags can be determined in an efficient manner.
TruSeq technology supports massively parallel sequencing using a proprietary reversible terminator-based method that enables detection of single bases as they are incorporated into growing DNA strands.
Published streptococcal and other 16S rRNA sequences were extracted from EMBL using proprietary extraction methods based on sequence profiles and annotation searches; representative sequences for each species were determined using a proprietary algorithm developed by SmartGene for bacterial 16S rRNA sequences (SmartGene IDNS™ Bacteria Module).
We have sequenced our tissue-specific libraries using a proprietary library preparation method, named Single Primer Isothermal Amplification (SPIA), which is ideal for use with mRNA-tagging, since the RNA yield from this approach is typically low [ 37, 66].
Sequence reads were aligned to the hg19 genome assembly using a proprietary Ion Proton pipeline (Life Technologies, USA).
Reference sequences were obtained from NCBI Entrez, and TaqMan assays designed using a proprietary primer-design module.
To ensure consistent mapping between datasets, clone/probe sequences were re-annotated against miRBase release 10.1 [ 19] using a proprietary Matlab (Mathworks Inc. Natick, MA, USA) script.
The miRNAs identified from cDNA sequencing of floral tissues, using the bioinformatics described above, were further verified using a proprietary microarray analysis with LC Sciences, in order to validate their expression in sexual and apomictic Boechera flower tissues.
Happy Host sets future rates using a proprietary algorithm.
Content is delivered from an international network of servers using a proprietary file transfer protocol.
