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The Wnt assay was performed using a procedure modified from the BPS Bioscience protocol.
We obtained naïve BMSCs from adult male Sprague Dawley rats (weight: 180 200 g) using a procedure modified from a previously described study [7].
Lipids were extracted from tissue samples using a procedure modified from that of Folch [8].
Catalysis by TMG was carried out using a procedure modified from Schuchardt and Lopes [ 14].
Dose response curves were determined using a procedure modified from Allemeersch et al. [ 41].
Average gene analysis was done using a procedure modified from [ 18].
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One in four sections was stained for myelin using a standard procedure modified from Woelcke [52], yielding an effective through-plane spacing of 200 µm.
The differences across all the three sites in the relationship between overall X-ray density and growth traits (see below) indicated that the Quesnel site is most favorable while PGTIS least favorable for growing interior spruce (YA El-Kassaby, pers. obs).. DNA extraction was performed on dormant vegetative buds of the sampled trees using a CTAB procedure modified after Doyle and Doyle [ 56].
Stefurak and Boynton (1986) do not find a difference in performance in trials in which the irrelevant dimension was modified using a procedure very similar to ours.
Genomic DNA was extracted from fresh cortex tissue of one P. pringlei cactus growing near La Paz, Baja California Sur, Mexico (23.985°N, 110.187°W), using a procedure with cetyltrimethylammonium bromide (CTAB) modified for mucilaginous cactus tissues (de la Cruz et al., 1997).
We use a procedure of a modified Gauss elimination method for solving this difference scheme in the case of one-dimensional fractional parabolic partial differential equations.
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