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A tenfold AuNP concentrate was processed under an N2 atmosphere to assess the activated partial thromboplastin time (aPTT) using a procedure adapted from our previous report [17].
Nuclear extracts were prepared using a procedure adapted from Lee et al. (Lee et al., 1988).
Hemichannel activity was measured using a procedure adapted from Orellana et al. (Orellana et al., 2009).
Dissected ovules from Arabidopsis thaliana plants were arranged around a cut style using a procedure adapted from [ 7] (see Methods).
Rheological measurements of the hydrogels were performed with an ARG2 Rheometer using a procedure adapted from a previous study (Zuidema et al., 2014).
Total Hg concentrations in blood and total and inorganic Hg concentrations in brain were measured using a procedure adapted from Greenwood et al. (1977).
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For the subcellular and PSD fractionation, five mouse brains were used in a procedure adapted from Huttner et al. [49].
RNA was isolated using a procedure specially adapted for laser capture microdissection samples (Stratagene Absolutely RNA Microprep kit, La Jolla, CA, USA).
The hydrolysis of triolein used as test reaction was performed following a procedure adapted from Rocha et al. (1999).
Cell wall thickness was measured directly from the CLSM images by a procedure adapted from those used previously to measure cell wall thickness in wood [ 16, 17].
Covariates were selected in the multivariate analysis using a stepwise procedure adapted to multiple imputation methodology [ 29].
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