These sediment samples were evaluated using a previously established sequential extractions procedure.
We analyze the time evolution of non-uniform spacing distributions using a previously established semi-empirical "spacing-diffusion" law [Akamatsu et al., Metal. Mater. Trans. A 35 (2004 18155].
For this purpose, adult porcine retinal explants were cultured using a previously established standard protocol for up to 5 days with specific TRPV4 agonist GSK1016790A (GSK), or specific antagonist RN-1734, or culture medium only.
Self-assembled constructs were grown for 4 weeks using a previously established method and were subjected to continuous 7-day treatment with 30 mM ribose during culture weeks 1, 2, 3, or 4, or for the entire 4-week culture.
The aim of the study was to examine the association of post-discharge AEs with timely follow-up visits after hospital discharge based on structured telephone interviews, health record review, and adjudication by two blinded, trained physicians using a previously established methodology.
Extrapulmonary lung injury was induced using a previously established double-hit model of ischemia reperfusion and fecal peritonitis [11, 12, 15].
DNA extraction was carried out using a previously established protocol [6] DNA was extracted from the portion of the specimen not used for microbiological culture as follows.
The esterase activity was calculated using a previously established standard curve with a fluorescein concentration range of 1 to 32 μmol/L (Fluka, Buchs, Switzerland).
2OH-CB3 was easily separated from 3OH-CB3 and 4OH-CB2 using a previously established LC-MS method, which allowed confirmation of the second peak (figure 2) to be 2OH-CB3 based on the retention time.
Mean global cortical PiB uptake relative to cerebellum expressed as SUVR was calculated in 20 AD patients and in 15 healthy human control subjects using a previously established VOI-based approach [48,49].
Using a previously established DNA-based predictor of age known as the "epigenetic clock," researchers found that a cohort of highly-traumatized African Americans were more likely to show aging-associated biochemical signatures in their DNA's epigenetic clock regions at an earlier age than what would otherwise be predicted by their chronological age.
