From the cylinders, tissue slices with a thickness of 200μm were prepared in cold PBS using a precision cutting tissue slicer (Krumdieck, Alabama Research and Development Corp., Munsford, USA).
Four cores of 0.6 mm were taken from each donor block and arrayed on a recipient paraffin block using a precision instrument (Tissue Arrayer, Beecher Instruments, Silver Spring, MD, USA).
Tissue cylinders of diameter of 1 mm were punched from the marked areas on each block and incorporated into a recipient paraffin block using a precision instrument, the tissue arrayer (Beecher Instruments, Silver Spring, Maryland, USA).
The powdered tissue was than portioned to 2 mg portions in 2 ml reaction tubes (Sarstedt, Germany) using a precision gauge (R200D, Sartorius, Germany).
Tissue cylinders with a diameter of 1.0 mm were punched from the marked areas of all blocks and brought into a recipient paraffin block using a precision instrument, as previously described [ 38].
Tissue cylinders with a diameter of 1.0 mm were punched from the specimen block and brought into an empty paraffin block [ 8] by using a precision instrument (Beecher Instruments, Silver Spring, MD, USA).
Since it is very simple and accurate to determine the tissue weight just before RNA extraction, this may be the method of choice, since there is only a small weighing error (0.2-0.6 0.2-0.6using a precision weighing device.
Five tissue cores of 0.6-mm diameter in each of the three areas mentioned above were placed into a recipient block by using a precision arraying instrument (Beecher Instruments; Micro Tissue Arrayer, Silver Springs, MD, USA).
Use a precision knife to do this.
TMAs were constructed using a custom-built precision Tissue Arrayer from Beecher Instruments (Silver Springs, MD, USA).
