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One-millilitre suspensions containing the required concentration of IJs were distributed across the soil surface of each arena using a precision pipette.
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Then, 1 mL release medium was removed with a precision pipette and sterile tips at 2, 4, 6, 24, 48, and 96 hours.
Use a precision knife to do this.
As previously described [39], cells were impaled with sharp microelectrodes filled with 2M KMeSO4 and 10mM HEPES, pH 7.4 (tip resistance 108.2±4.7 MΩ), pulled from borosilicate glass capillaries (World Precision Instruments, Sarasota, FL) using a P80 pipette puller (Sutter Instruments, Novato, CA).
Using a long pipette, the technician distributed the slurry among five sterilized plastic bottles.
It is immobilized using a holding pipette.
The top phase was carefully separated using a Pasteur pipette.
In parallel, 0.1 mL was decanted into Delvotest SP-NT ampoules using a specific pipette for each sample.
We then implemented a controlled indentation scheme using a glass pipette to mechanically stimulate individual neurites that were adjacent to the pipette.
Patch electrodes were pulled from borosilicate glass (Hilgenberg, Malsfeld, Germany) using a horizontal pipette puller (Zeitz Instruments, Augsburg, Germany) to yield pipettes with a resistance of 3 6 MΩ.
A scratch wound was made the next day using a plastic pipette tip.
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