Absorbance was read at 405 nm using an Emax precision microplate reader (Molecular devices, Sunnyvale, USA).
After 2 hours, the absorbance at 450 nm was measured using an Emax Precision Microplate Reader (Molecular Devices, Sunnyvale, CA, USA).
After incubation, 120 μl of glycine (0.2 M, pH 10.7) was added to each well, and the absorbance at 405 and 490 nm was measured using an Emax Precision Microplate Reader (Molecular Devices, Sunnyvale, CA, USA).
Use a precision knife to do this.
Absorbance at 550 nm was measured using a microplate spectrophotometer system (Microplate Reader 2001, BioWhittaker) and results were presented as a ratio of control values.
Lastly, absorbance was measured at 490 nm using a microplate reader (Bio-Tek Synereader micreadere reader).
Absorbance at 540 nm was measured using a microplate reader (iMark microplate reader; Bio-Rad).
After incubation, the absorbance was measured at 492 nm using a microplate reader (318C-Microplate Reader, China).
Reaction was stopped adding 50 µl of 1 M H2SO4 and measured at 490 nm using the Emax Precision Microplate Reader, Molecular Devices.
